CElixir™ family of products has been optimized to perform best when used with positively charged or amphoteric negatively charged, neutral compounds or mixtures of all.

Although most parameters affecting CElixir™ CZE will be the same as when used with Bare Fused Silica; CZE with CElixir™ is different. It is important that you NOT simply substitute CElixir™ into your current method for your run buffer. To get the most out of CElixir’s features, you should develop a new method and follow our method development steps listed below. You should find the pH at which your sample’s separation is best with CElixir™.

Method Development Steps

1. Following the instructions in the CElixir™ Operating and Trouble Shooting Manual, select the CElixir™ Accelerator Solution (B) that is two points lower than your analyte’s pK or pI. If you do not know the pK or pI, start with Accelerator Solution (B) pH 2.5. Include in this run, a neutral marker to determine EOF along with the migration time of your peaks.
2. Run the same method as above but this time dissolve your sample in the next higher pH of CElixir™ Accelerator Solution (B). Note EOF and migration time of your peaks.
3. Repeat the above step with each available Accelerator Solution (B) until you operate with CElixir™ at pH 9.2.
4. Find two runs where you achieved your best resolution and efficiency. It is most likely that your optimized run is using CElixir™ at the pH somewhere between these two runs.
5. Follow the instructions on page 24 of the CElixir™ Operating and Trouble Shooting Manual to adjust the Accelerator Solution (B). Continue to bracket the pK or suspected pK until you are satisfied with the results.