Easy to Apply Instructions

Using CElixir™ is very simple and robust. A complete Operating and Trouble Shooting Manual is included with every column kit and our help desk is available to assist you in your method development. Using CElixir™ is very simple; once you have followed the instrument manufacturer’s instructions to stabilize the instrument, prepare your sample in CElixir™ Accelerator Solution (B) of the pH of your choice, then;

See Important Information for Prospective CElixir™ Users

Beginning Steps to use CElixir™

1. Condition Your Capillary. Run 0.1N NaOH solution through the capillary for at least one minute at 2Bar. Inlet vial NaOH, outlet vial to waste.
2. Rinse the Capillary. Run CEwater™ through the capillary for two minutes. Inlet vial CEwater, outlet vial to waste; 2Bar.
3. Apply the Initial Coating of the capillary with Initiator Solution (A). Run Initiator through the capillary for at least 1 minute. Inlet vial CElixir Initiator Solution, outlet vial waste; 2Bar.
4. Apply the Final Coating of the capillary with Accelerator Solution (B). Run Accelerator (B) of the pH you have chosen for your sample for two minutes. Inlet vial CElixir Accelerator (B), pH of your choice, outlet vial waste; 2Bar.
5. Inject your sample. Inject approximately 2% of your capillary volume with cathodic vial. Inlet vial Sample, outlet vial CElixir Accelerator (B), pH of your choice. Different vial from Step 4.
6. Insert a CEwater™ plug by injecting it for 1 second. Inlet vial CEwater, outlet vial CElixir Accelerator (B), same as Step 5; 50mBar for 1 sec.
7. From a different vial (not the one used in Step 4) run a vial with Accelerator Solution (B) into a vial with Accelerator Solution (B). Inlet vial Accelerator Solution (B), pH of your choice, outlet vial Accelerator (B), pH of your choice.
8. Repeat steps 1-7 between each injection.

Final Steps for CElixir™ and care for the capillary

1. Fill the Capillary with 0.1N NaOH and let it stand for at least one minute.
2. Rinse the Capillary for at least one minute with 0.1N HCL.
3. Rinse the Capillary for at least one minute with 0.1N NaOH.
4. Rinse the Capillary for at least one minute with CEwater™.
5. Blow dry, air or nitrogen through the capillary till dry.

View our Easy to Use Instruction Card