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Parameters for CElixir Methods using an Agilent 3D Capillary Electrophoresis Instrument
Easy to Apply Instructions
It is very easy to set up a method in the Agilent 3D CE Instrument. Using the following parameters, enter
information into your unit using our sample method.
Method Information: Generic CElixir Method
| Run Time Checklist: |
Pre run Cmd/Macro:
Data Acquisition:
Standard Data Analysis:
Customized Data Analysis:
Save GLP Data:
Post run Cmd/Macro:
Save Method with Data: |
Off
On
Off
Off
Off
Off
On |
HPCE Mode: CE+p
| Home Values: |
Lift Offset:
Cassette Temperature:
Inlet Home Vial:
Outlet Home Vial: |
4
25.00ºC
4, CElixir Accelerator
5, CElixir Accelerator |
Replenishment and Pre-conditioning:
Selected: Serial Processing
Replenishment Entries: No replenishment used
| Pre-conditioning Entries: |
|
Function |
Parameter |
|
1. HIFLUSH |
2.0 bar, 0.0 kV, 1 minute
Inlet: Vial #1 (0.1N NaOH)
Outlet: Vial #7 (waste) |
|
2. HIFLUSH |
2.0 bar, 0.0 kV, 1.00 minute
Inlet: Vial #2 (CElixir Initiator Solution (A))
Outlet: Vial #7 (waste) |
|
3. HIFLUSH |
2.0 bar, 0.0 kV, 2.00 minutes
Inlet: Vial #3 (CElixir Accelerator Solution (B))
Outlet: Vial #7 (waste) |
Post Condition Entries: No Post conditioning used
| Electric: (limits) |
Electric:
Polarity:
Voltage:
Current:
Power:
Low Current Limit: |
On
Positive
15.00 kV
100.0µA
System Limit
0.0µA (can be set to 0.1µ) |
| Injection Table Entries: |
|
Function |
Parameter |
|
1. Pressure |
50.0 mbar, 5.0 seconds,
Inlet: Vial # N*: Injection Vial
Outlet Vial; 5: CElixir Accelerator
*Can be any vial number with dissolved analytes in
them. If you dissolve in CEwater you will enjoy benefits
of stacking. If you dissolve samples in CElixir you will not. |
|
2. Pressure |
50.0 mbar, 1.0 second,
Inlet: Vial # 6 (Water)
Outlet: Vial# 5: CElixir Accelerator |
| Store Data Options: |
Collect Voltage:
Collect Current:
Collect Power:
Collect Pressuret:
Collect Temperature: |
No
Yes
No
No
No |
| Time Entries: |
Stop time:
Post time: |
25.00 minutes
Off |
| Time Table: |
Time (minutes)
0.00
1.00 |
Function
Voltage
Voltage |
Parameter
0.00 kV
15.00 kV |
| Diode Array Detector Settings: |
Stop time:
Post time:
Response time:
Peak width:
Pre run Auto balance:
Post run Auto balance: |
25.00 minutes
Off
0.1 second
<0.01 minutes
On
Off |
Time Table: Empty (no settings provided)
| Vial Table Information for this Method: |
Vial#
1.
2.
3.
4.
5.
6.
7.
8-46 |
Used in
Any use
Any use
Any use
Any use
Any use
Any use
Any use
Any use |
Name
0.1N NaOH
CElixir Initiator (A)
CElixir Acc (B)
CElixir Acc (B)
CElixir Acc (B)
CEwater
Waste
Samples |
Comment
Sodium Hydroxide
For Flushing (1st part of double layer coating)
For Flushing (2nd layer of coating)
Inlet Vial
Outlet Vial
For injection plug
Waste
Samples |
Note:
1. The outlet vial is CElixir Accelerator Solution and this should be used during injection. This is very important
for good results.
2. All CElixir Accelerator Solutions (B) should be the same pH in any single run.
Fraction Collection: Off
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