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| Technical Data: |
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| Moisture: |
< 7% |
| EEO (electroendosmosis): |
< 0.13 -mr |
| Sulfate: |
< 0.20% |
| Gelling Temp. (at 1.5%): |
36° + 1.5°C |
| Melting Temp. (at 1.5%): |
88° + 1.5°C |
| Gel Strength (at 1.0%): |
1200g/cm2 |
| Gel Strength (at 1.5%): |
2500g/cm2 |
| DNase / RNase: |
None Detected |
| Protease: |
None Detected |
| Endonuclease: |
None Detected |
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Agarose LE™
High performance, general purpose agarose
- Multi-purpose, high purity
- Enhanced resolution and clarity
- Reduced background
- Low EEO / increased electrophoretic mobility
- RNase, DNase, Protease-free
- High hysteresis, improved thermal stability
Agarose LE (low electro endosmotic) is a highly purified agarose suitable for a variety of eletrophoeretic techniques and molecular biology applications. It is refined using an advanced process that
excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used
for analyses of proteins and nucleic acids of various sizes (150 bp to 6 kbp).
Its low EEO (< 0.13-m) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows
macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO
also provides a reduction in band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.
Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion)
and various blotting protocols.
It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colorless solution at 1g:100ml H O), and exhibits
exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.
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